Effect of Oenothera odorata Root Extract on Microgravity and Disuse-Induced Muscle Atrophy

Figure 2

Effect of EVP on HSP70 expression by microgravity in C2C12 myoblasts. (a) Effect of microgravity on HSP70 expression. After culturing with media for 24 hr, myoblasts were subjected to 3D-clinorotation for 0–24 hr. HSP70 and β-actin were analyzed by immunoblot analysis using specific antibodies. (b) Effect of EVP on microgravity by 3D-clinostat. C2C12 myoblasts were cultured in 12-well plates until confluent, and the medium was then replaced to serum-free medium with or without the EVP (50 μg/mL) for 24 hr. 3D-clinorotation subjected for 24 hr with DMEM containing 10% (v/v) FBS. Protein lysate was extracted using the PRO-PREP Protein extraction Kit. The whole lysates were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 12% polyacrylamide gels. HSP70 and β-actin were analyzed by immunoblot analysis using specific antibodies. Immunoblot was analyzed by densitometry and the inserts display representative blots of four similar independent experiments, respectively.

(a)

(b)