Effect of Oenothera odorata Root Extract on Microgravity and Disuse-Induced Muscle Atrophy

Figure 6

Effect of EVP on HSP70 expression by disuse muscle atrophy in mice. GM of sciatic denervated mice were compared with control and EVP groups. Protein lysate was extracted using the PRO-PREP Protein extraction Kit. The whole lysates were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on 12% polyacrylamide gels. HSP70 and β-actin were analyzed by immunoblot analysis using specific antibodies. Immunoblot was analyzed by densitometry and the inserts display representative blots of four similar independent experiments, respectively.