Table of Contents Author Guidelines Submit a Manuscript
Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 132706, 7 pages
http://dx.doi.org/10.1155/2015/132706
Research Article

Aquapuncture Using Stem Cell Therapy to Treat Mdx Mice

1Stem Cell Lab, Surgery Department, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Avenue, 05508-270 São Paulo, SP, Brazil
2Center for Cellular and Molecular Studies and Therapy (NETCEM), São Paulo, SP, Brazil
3Clinic Department, School of Veterinary Medicine, University of São Paulo, 87 Prof. Dr. Orlando Marques de Paiva Avenue, 05508-270 São Paulo, SP, Brazil
4Nuclear and Energy Research Institute Biotechnology Center, 2242 Prof. Lineu Prestes Avenue, 05508-270 São Paulo, SP, Brazil
5Division of Chinese Medicine and Acupuncture, Federal University of São Paulo, 862 Botucatu Street, 04023-900 São Paulo, SP, Brazil
6Obstetrics Department, School of Arts, Sciences and Humanities, University of São Paulo, 1000 Arlindo Bettio Avenue, 03828-000 São Paulo, SP, Brazil

Received 2 March 2015; Accepted 19 April 2015

Academic Editor: Andreas Sandner-Kiesling

Copyright © 2015 Greyson Vitor Zanatta Esper et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Duchenne muscular dystrophy (DMD) occurs due to genetic mutations that lead to absence or decrease of dystrophin protein generating progressive muscle degeneration. Cell therapy using mesenchymal stem cell (MSC) has been described as a treatment to DMD. In this work, MSC derived from deciduous teeth, called stem cells from human exfoliated deciduous teeth (SHED), were injected in acupoint as an alternative therapy to minimize muscle degeneration in twenty-two mdx mice. The treatment occurred three times with intervals of 21 days, and animals were analyzed four times: seven days prior treatment (T-7); 10 days after first treatment (T10); 10 days after second treatment (T31); and 10 days after third treatment (T52). Animals were evaluated by wire test for estimate strength and blood was collected to perform a creatinine phosphokinase analysis. After euthanasia, cranial tibial muscles were collected and submitted to histological and immunohistochemistry analyses. Treated groups presented improvement of strength and reduced creatinine phosphokinase levels. Also, a slight dystrophin increase was observed in tibial cranial muscle when aquapuncture was associated SHED. All therapies have minimized muscle degeneration, but the association of aquapuncture with SHED appears to have better effect, reducing muscle damage, suggesting a therapeutic value.