Research Article

A Cistanches Herba Fraction/β-Sitosterol Causes a Redox-Sensitive Induction of Mitochondrial Uncoupling and Activation of Adenosine Monophosphate-Dependent Protein Kinase/Peroxisome Proliferator-Activated Receptor γ Coactivator-1 in C2C12 Myotubes: A Possible Mechanism Underlying the Weight Reduction Effect

Figure 6

Effects of HCF1 on PGC-1 nuclear translocation in C2C12 myotubes. Cells were preincubated with HCF1 for 8 h. The extent of PGC-1 nuclear translocation was determined by the partition of PGC-1 between cytosolic and nuclear fractions, as described in Materials and Methods section. Data are expressed as percent control with respect to non-HCF1 preincubated controls. Values given are means ± SEM, with . *Significantly different from the unpreincubated control group ().
(a)
(b)
(c)
(d)