Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

Figure 4

Effect of 17 compounds on (a) cell viability and LPS-induced (b) NO production in RAW 264.7 cells. RAW 264.7 cells were pretreated with 17 compounds for 1 hour before incubation with LPS for 24 hours. (a) Cytotoxicity was evaluated by a CCK. (b) The culture supernatant was analyzed for nitrite production. As a control, the cells were incubated with vehicle alone. Data shows mean ± SE values of triplicate determination from independent experiments. and were calculated from comparing with LPS-stimulation value.

(a)

(b)