Protective Effects of Total Saponins of Panax Notoginseng on Steroid-Induced Avascular Necrosis of the Femoral Head In Vivo and In Vitro

<div>TSPN attenuated apoptosis and reduced Caspase-3 expression in osteocytes in the model of steroid-induced ANFH. (a) TUNEL staining of femoral heads in each group (<svg height="7.66065pt" id="M16" style="vertical-align:-0.3625798pt" version="1.1" viewbox="-0.0498162 -7.29807 8.08224 7.66065" width="8.08224pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M528 54L331 254L528 455L492 493L294 291L96 493L60 455L257 254L60 54L96 16L294 217L492 16L528 54Z" id="g117-42"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g></svg>200). (b) Immunohistochemical determination of manifestation of Caspase-3 expression (<svg height="7.66065pt" id="M17" style="vertical-align:-0.3625798pt" version="1.1" viewbox="-0.0498162 -7.29807 8.08224 7.66065" width="8.08224pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0135,0,0,-0.0135,0,0)"><path d="M528 54L331 254L528 455L492 493L294 291L96 493L60 455L257 254L60 54L96 16L294 217L492 16L528 54Z" id="g117-42"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="587" vert-adv-y="587"></glyph.data></g></svg>400). The positive osteocytes are indicated shown with black arrows.</div>

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Figure 2

Figure 2: Protective Effects of Total Saponins of Panax Notoginseng on Steroid-Induced Avascular Necrosis of the Femoral Head In Vivo and In Vitro 