The Flavonoid Apigenin Ameliorates Cisplatin-Induced Nephrotoxicity through Reduction of p53 Activation and Promotion of PI3K/Akt Pathway in Human Renal Proximal Tubular Epithelial Cells

Figure 5

The effect of wortmannin on cisplatin-induced apoptosis in HK-2 cells. Cells were pretreated with or without 100 nM of wortmannin for 1 h and then exposed to 40 μM of cisplatin for 8 h (a) or 24 h (b and c). The phosphorylation levels of p53 and Akt were examined by Western blot analysis using anti-phospho-p53 (Ser15) and anti-phospho-Akt (Ser473) antibodies. Expression of total p53 and Akt was also identified using anti-p53 and anti-Akt antibodies (a). Caspase-3 activity was measured using a caspase-3 colorimetric assay kit. Values are means ± SD, . versus cells treated with cisplatin alone (b). The levels of cleaved caspase-3 and PARP were examined by Western blot analysis using anticleaved caspase-3 (p20) and anti-PARP antibodies (c). The density of band was quantified by a densitometry using Bio-Rad Quantity One software. Value are means ± SD, . versus cells treated with cisplatin alone.

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