Vitex agnus-castus L. (Verbenaceae) Improves the Liver Lipid Metabolism and Redox State of Ovariectomized Rats

Figure 6

Evaluation of the liver redox status. The liver oxidative status was evaluated by assessing the liver GSH levels. GSH (panel (a), μg GSH/mg protein; ); liver lipid peroxidation using the TBARS method (panel (b), nmol MDA/mg protein; ); total Cu,ZnSOD activity (panel (c)) and KCN-sensitive Cu,ZnSOD (panel (d)) were measured and expressed as U SOD/mg protein (); CAT activity (panel (e), μmol H₂O₂ consumed/min × mg protein; ); GR activity (panel (f), NADPH oxidized/min × mg protein; ); G6PD activity (panel (g), NADPH produced/min × mg protein; ) and GPx3 activity (panel (h), NADPH oxidized/min × mg protein; ) were evaluated. The vertical bars represent the standard error. The letters indicate significant differences between the values as determined using ANOVA ( OVX versus control, OVX + CE, and OVX + ButF).

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