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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 235265, 9 pages
Research Article

Icariin Prevents Amyloid Beta-Induced Apoptosis via the PI3K/Akt Pathway in PC-12 Cells

1Department of Integrated Chinese and Western Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China
2Department of Traditional Chinese Medicine, Zhong Shan Hospital Xiamen University, Xiamen, Fujian 361004, China

Received 25 November 2014; Accepted 5 January 2015

Academic Editor: Ki-Wan Oh

Copyright © 2015 Dongdong Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1: Protective effects of icariin on Aß25–35 induced apoptosis in PC12 cells.(I) Pro-apoptotic effect of Aß25–35. After treatment with the following concentrations of Aß25–35,cell apoptosis was evaluated by AnnexinV/PI staining. (II) Protective effect of icariin against Aß25-35-induced apoptosis. After 1h pretreatment with icariin (0, 2.5, 5, 10,or 20 µM), PC12 cells were treated with 20 µM Aß25–35 for 24h. Apoptosis was evaluated by AnnexinV/PI staining. Results are presented as the mean ±S.D. of five independent experiments.**p <0.01 versus Aß25–35 treatment; # p <0.05 versus untreated control.

  1. Supplementary Material