Strong Manual Acupuncture Stimulation of “Huantiao” (GB 30) Reduces Pain-Induced Anxiety and p-ERK in the Anterior Cingulate Cortex in a Rat Model of Neuropathic Pain

Figure 4

Double immunofluorescence localizing p-ERK in ACC neurons, macrophages, and astrocytes in the ACC after L5 spinal nerve ligation. ((a)–(e)) p-ERK (red) colocalizes with NeuN (green); ((f)–(j)) p-ERK (red) does not colocalize with OX-42 (green); ((k)–(o)) p-ERK (red) does not colocalize with GFAP (green).

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