Protective Effects of Scutellarin on Human Cardiac Microvascular Endothelial Cells against Hypoxia-Reoxygenation Injury and Its Possible Target-Related Proteins
Table 1
Differentially-expressed proteins found in proteomic analysis of cytoplasm protein expression profiles of different groups and results of MS/MS identification.
Spot
Target protein name
Accession number of NCBI database
Theoretical Mr (Da)/pI
Protein score
Sequence coverage (%)
Best ion score
Spot volume (ppm)
Fold change
CT group
HR group
HR/CT
1
Chain A, 14-3-3 sigma in complex with Yap Ps127-peptide
306991738
26501/4.85
236
41
68
333.96 ± 223.4
172.3 ± 108.6
0.52
2
Chain B, crystal structure of human Nudt5 complexed with 8-oxo-Dgmp
317455160
21604/4.94
92
15
62
11.99 ± 14.9
1.36 ± 2.63
0.11
3
Glutathione S-transferase P
121746
23341/5.43
328
53
136
77 ± 71.7
27.6 ± 29.0
0.36
4
CGI-114 protein
5817183
26844/6.41
93
30
48
7.28 ± 6.2
2.4 ± 2.9
0.33
5
Inorganic pyrophosphatase
8247940
32639/5.54
179
34
66
36.88 ± 30.0
14.7 ± 8.3
0.40
6
COP9 complex subunit 4
5410300
46169/5.57
242
39
89
5.57 ± 4.79
2.26 ± 1.8
0.41
7
Heat shock protein
386785
69825/5.42
200
22%
59
52.9 ± 42.3
16.1 ± 17.9
0.30
CT group
SCU group
SCU/CT
8
Heat shock 60 kDa protein 1 (chaperonin), isoform CRA_c
