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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 287153, 13 pages
http://dx.doi.org/10.1155/2015/287153
Research Article

Aqueous Extract of Agaricus blazei Murrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats

1Department of Morphological Sciences, State University of Maringá (UEM), 87020-900 Maringá, PR, Brazil
2Center of Biological Sciences, State University of the North of Paraná (UENP), Bandeirantes, PR, Brazil
3Department of Biochemistry, State University of Maringá (UEM), 87020-900 Maringá, PR, Brazil

Received 8 October 2014; Revised 15 December 2014; Accepted 16 December 2014

Academic Editor: Chunchao Han

Copyright © 2015 Ana Paula de Santi-Rampazzo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. L. Tiana, Q. Caib, and H. Wei, “Alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging,” Free Radical Biology & Medicine, vol. 24, no. 9, pp. 1477–1484, 1998. View at Publisher · View at Google Scholar · View at Scopus
  2. T. A. Prolla and M. P. Mattson, “Molecular mechanisms of brain aging and neurodegenerative disorders: lessons from dietary restriction,” Trends in Neurosciences, vol. 24, pp. 21–31, 2001. View at Publisher · View at Google Scholar · View at Scopus
  3. A. M. Vincent, J. W. Russell, P. Low, and E. L. Feldman, “Oxidative stress in the pathogenesis of diabetic neuropathy,” Endocrine Reviews, vol. 25, no. 4, pp. 612–628, 2004. View at Publisher · View at Google Scholar · View at Scopus
  4. H. Imai and Y. Nakagawa, “Biological significance of phospholipid hydroperoxide glutathione peroxidase (PHGPx, GPx4) in mammalian cells,” Free Radical Biology & Medicine, vol. 34, no. 2, pp. 145–169, 2003. View at Publisher · View at Google Scholar · View at Scopus
  5. T. Takaku, Y. Kimura, and H. Okuda, “Isolation of an antitumor compound from Agaricus blazei Murill and its mechanism of action,” Journal of Nutrition, vol. 131, no. 5, pp. 1409–1413, 2001. View at Google Scholar · View at Scopus
  6. K. E. Hall, “Aging and neural control of the GI tract II. Neural control of the aging gut: can an old dog learn new tricks?” The American Journal of Physiology—Gastrointestinal and Liver Physiology, vol. 283, no. 4, pp. 827–832, 2002. View at Google Scholar · View at Scopus
  7. P. R. Wade, “Aging and neural control of the GI tract I. Age-related changes in the enteric nervous system,” The American Journal of Physiology—Gastrointestinal and Liver Physiology, vol. 283, no. 3, pp. G489–G495, 2002. View at Google Scholar · View at Scopus
  8. A. C. M. Marese, P. de Freitas, and M. R. M. Natali, “Alterations of the number and the profile of myenteric neurons of Wistar rats promoted by age,” Autonomic Neuroscience: Basic & Clinical, vol. 137, no. 1-2, pp. 10–18, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. P. Höhn, H. Gabbert, and R. Wagner, “Differentiation and aging of the rat intestinal mucosa. II. Morphological, enzyme histochemical and disc electrophoretic aspects of the aging of the small intestinal mucosa,” Mechanisms of Ageing and Development, vol. 7, no. 3, pp. 217–226, 1978. View at Publisher · View at Google Scholar · View at Scopus
  10. F. Raul, F. Gosse, M. Doffoel, P. Darmenton, and J. Y. Wessely, “Age related increase of brush border enzyme activities along the small intestine,” Gut, vol. 29, no. 11, pp. 1557–1563, 1988. View at Publisher · View at Google Scholar · View at Scopus
  11. J. B. Furness, M. Costa, A. Rökaeus, T. J. McDonald, and B. Brooks, “Galanin-immunoreactive neurons in the guinea-pig small intestine: their projections and relationships to other enteric neurons,” Cell and Tissue Research, vol. 250, pp. 607–615, 1987. View at Google Scholar
  12. C. Thrasivoulou, H. Ridha, V. Soubeyre, C. H. V. Hoyle, M. J. Saffrey, and T. Cowen, “Free radical buffering in rat enteric neurons: effects of age and neurotrhophic factors,” European Journal of Neuroscience, vol. 12, p. 33, 2000. View at Google Scholar
  13. N. F. Dolatshad, A. T. Silva, V. Soubeyre, C. H. V. Hoyle, T. Cowen, and M. J. Saffrey, “Expression of the GNDF and its receptors in the ageing gut,” Soc Neurosci Abtsr 27:861–864, 2001.
  14. J. P. F. Schoffen and M. R. M. Natali, “Effect of age on the myosin-V immunoreactive myenteric neurons of rats ileum,” Biocell, vol. 31, no. 1, pp. 33–39, 2007. View at Google Scholar · View at Scopus
  15. A. A. Soares, C. G. M. de Souza, F. M. Daniel, G. P. Ferrari, S. M. G. da Costa, and R. M. Peralta, “Antioxidant activity and total phenolic content of Agaricus brasiliensis (Agaricus blazei Murril) in two stages of maturity,” Food Chemistry, vol. 112, no. 4, pp. 775–781, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. A. A. Soares, Efeitos do cogumelo Agaricus brazei Murrill sobre os danos causados pelo paracetamol aos tecidos hepático e cerebral de ratos [Ph. D. thesis], University of Maringa, Maringá, Brazil, 2013.
  17. O. Erel, “A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation,” Clinical Biochemistry, vol. 37, no. 4, pp. 277–285, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. I. F. Tannock, “A comparison of the relative efficiencies of various metaphase arrest agents,” Experimental Cell Research, vol. 47, no. 1-2, pp. 345–356, 1967. View at Publisher · View at Google Scholar · View at Scopus
  19. Z. Lin, N. Gao, H.-Z. Hu et al., “Immunoreactivity of Hu proteins facilitates identification of myenteric neurones in guinea-pig small intestine,” Neurogastroenterology & Motility, vol. 14, no. 2, pp. 197–204, 2002. View at Publisher · View at Google Scholar · View at Scopus
  20. H. F. Wrzos, A. Cruz, R. Polavarapu, D. Shearer, and A. Ouyang, “Nitric oxide synthase (NOS) expression in the myenteric plexus of streptozotocin-diabetic rats,” Digestive Diseases and Sciences, vol. 42, no. 10, pp. 2106–2110, 1997. View at Publisher · View at Google Scholar · View at Scopus
  21. R. J. Phillips, E. J. Kieffer, and T. L. Powley, “Loss of glia and neurons in the myenteric plexus of the aged Fischer 344 rat,” Anatomy and Embryology, vol. 209, no. 1, pp. 19–30, 2004. View at Publisher · View at Google Scholar · View at Scopus
  22. D. M. G. Sant'ana, M. H. de Miranda-Neto, R. R. de Souza, and S. L. Molinari, “Morphological and quantitative study of the myenteric plexus of the ascending colon of rats subjected to proteic desnutrition,” Arquivos de Neuro-Psiquiatria, vol. 55, no. 4, pp. 687–695, 1997. View at Publisher · View at Google Scholar · View at Scopus
  23. T. Jayakumar, P. Aloysius Thomas, and P. Geraldine, “Protective effect of an extract of the oyster mushroom, Pleurotus ostreatus, on antioxidants of major organs of aged rats,” Experimental Gerontology, vol. 42, no. 3, pp. 183–191, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Gommers, M. Dehez-Delhaye, and D. J. Caucheteux, “Morphological and metabolic alterations in adipose tissue of very old rats,” Journals of Gerontology, vol. 38, no. 6, pp. 666–672, 1983. View at Google Scholar · View at Scopus
  25. M. R. Caccia, J. B. Harris, and M. A. Johnson, “Morphology and physiology of skeletal muscle in aging rodents,” Muscle and Nerve, vol. 2, no. 3, pp. 202–212, 1979. View at Publisher · View at Google Scholar · View at Scopus
  26. E. Perissinotto, C. Pisent, G. Sergi, F. Grigoletto, and G. Enzi, “Anthropometric measurements in the elderly: age and gender differences,” The British Journal of Nutrition, vol. 87, no. 2, pp. 177–186, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. B. Steen, “Body composition and aging.,” Nutrition Reviews, vol. 46, no. 2, pp. 45–51, 1988. View at Google Scholar · View at Scopus
  28. R. J. Phillips, E. J. Kieffer, and T. L. Powley, “Aging of the myenteric plexus: neuronal loss is specific to cholinergic neurons,” Autonomic Neuroscience: Basic and Clinical, vol. 106, no. 2, pp. 69–83, 2003. View at Publisher · View at Google Scholar · View at Scopus
  29. R. J. R. Johnson, M. Schemann, R. M. Santer, and T. Cowen, “The effects of age on the overall population and on sub-populations of myenteric neurons in the rat small intestine,” Journal of Anatomy, vol. 192, no. 4, pp. 479–488, 1998. View at Publisher · View at Google Scholar · View at Scopus
  30. M. R. C. G. Novaes, “Cogumelos comestíveis da ordem Agaricales: Aspectos nutricionais e atividade farmacológica no câncer,” Infarma, vol. 19, pp. 147–150, 2007. View at Google Scholar
  31. P. Parini, B. Angelin, and M. Rudling, “Cholesterol and lipoprotein metabolism in aging: reversal of hypercholesterolemia by growth hormone treatment in old rats,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 19, no. 4, pp. 832–839, 1999. View at Publisher · View at Google Scholar · View at Scopus
  32. R. A. Kreisberg and S. Kasim, “Cholesterol metabolism and aging,” The American Journal of Medicine, vol. 82, no. 1, supplement 2, pp. 54–60, 1987. View at Publisher · View at Google Scholar · View at Scopus
  33. A. Ledwoż;yw, J. Michalak, A. Stępień, and A. Ka̧dziołka, “The relationship between plasma triglycerides, cholesterol, total lipids and lipid peroxidation products during human atherosclerosis,” Clinica Chimica Acta, vol. 155, no. 3, pp. 275–283, 1986. View at Publisher · View at Google Scholar · View at Scopus
  34. D. Q.-H. Wang, “Aging per se is an independent risk factor for cholesterol gallstone formation in gallstone susceptible mice,” The Journal of Lipid Research, vol. 43, no. 11, pp. 1950–1959, 2002. View at Publisher · View at Google Scholar · View at Scopus
  35. C. Gälman, M. Matasconi, L. Persson, P. Parini, B. Angelin, and M. Rudling, “Age-induced hypercholesterolemia in the rat relates to reduced elimination but not increased intestinal absorption of cholesterol,” The American Journal of Physiology—Endocrinology and Metabolism, vol. 293, no. 3, pp. E737–E742, 2007. View at Publisher · View at Google Scholar · View at Scopus
  36. M. G. Ahmed, E. Y. Heba, and H. I. Hala, “Protective effects of mushroom and their ethyl extract on aging rats compared with L-carnitine,” Nature, vol. 8, no. 10, 2010. View at Google Scholar
  37. S. C. Jeonga, Y. T. Jeongb, and B. K. Yangb, “White button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats,” Nutrition Research, vol. 30, pp. 49–56, 2010. View at Google Scholar
  38. Y. Liu, Y. Fukuwatari, K. Okumura et al., “Immunomodulating activity of Agaricus brasiliensis KA21 in mice and in human volunteers,” Evidence-Based Complementary and Alternative Medicine, vol. 5, no. 2, pp. 205–219, 2008. View at Publisher · View at Google Scholar · View at Scopus
  39. A. F. Eira, Cultivo do cogumelo medicinal Agaricus blazei (Murril) ss. Heinemann ou Agaricus brasiliensis (Wasser et al.), Aprenda Fácil, Viçosa, Brazil, 2003.
  40. E. V. Hillyer and K. E. Quesenberry, Ferrets, Rabbits and Rodents Clinical Medicine and Sugery, Saunders, London, UK, 1997.
  41. W. Liu, W. Yue, and R. Wu, “Effects of diabetes on expression of glial fibrillary acidic protein and neurotrophins in rat colon,” Autonomic Neuroscience: Basic & Clinical, vol. 154, no. 1-2, pp. 79–83, 2010. View at Publisher · View at Google Scholar · View at Scopus
  42. L. E. Rikans, “Influence of aging on the susceptibility of rats to hepatotoxic injury,” Toxicology and Applied Pharmacology, vol. 73, no. 2, pp. 243–249, 1984. View at Publisher · View at Google Scholar · View at Scopus
  43. I. P. B. Moreira, L. E. B. Amado, A. L. F. Bersani, C. A. Bersani-Amado, and S. M. Caparroz-Assef, “Principais aspectos do tratamento das infecções no idoso,” Ciência, Cuidado e Saúde, vol. 6, pp. 488–495, 2007. View at Google Scholar
  44. Y.-L. Lee, S.-Y. Jian, P.-Y. Lian, and J.-L. Mau, “Antioxidant properties of extracts from a white mutant of the mushroom Hypsizigus marmoreus,” Journal of Food Composition and Analysis, vol. 21, no. 2, pp. 116–124, 2008. View at Publisher · View at Google Scholar · View at Scopus
  45. L. F. Barbisan, M. Miyamoto, C. Scolastici et al., “Influence of aqueous extract of Agaricus blazei on rat liver toxicity induced by different doses of diethylnitrosamine,” Journal of Ethnopharmacology, vol. 83, no. 1-2, pp. 25–32, 2002. View at Publisher · View at Google Scholar · View at Scopus
  46. A. M. Al-Dbass, S. K. Al- Daihan, and R. S. Bhat, “Agaricus blazei Murill as an efficient hepatoprotective and antioxidant agent against CCl4-induced liver injury in rats,” Saudi Journal of Biological Sciences, vol. 19, no. 3, pp. 303–309, 2012. View at Publisher · View at Google Scholar · View at Scopus
  47. J. L. Quiles, J. J. Ochoa, M. Battino et al., “Life-long supplementation with a low dosage of coenzyme Q10 in the rat: effects on antioxidant status and DNA damage,” BioFactors, vol. 25, no. 1–4, pp. 73–86, 2005. View at Publisher · View at Google Scholar · View at Scopus
  48. W. B. Gartner and J. L. Hiat, Tratado de Histologia em cores, Elsevier, Rio de Janeiro, Brazil, 2007.
  49. F. Moog, “The small intestine in old mice: growth, alkaline phosphatase and disaccharidase activities, and deposition of amyloid,” Experimental Gerontology, vol. 12, no. 5-6, pp. 223–235, 1977. View at Publisher · View at Google Scholar · View at Scopus
  50. I. N. Valenkevich and N. M. Zhukova, “The structure of the mucous membrane of the human duodenum with aging,” Arkhiv Patologii, vol. 38, pp. 58–61, 1976. View at Google Scholar
  51. M. Macari, R. L. Furlan, and E. Gonzales, Fisiologia aviária aplicada a frangos de corte, São Paulo, Brazil, Funep, 2002.
  52. J. R. Pluske, D. J. Hampson, and I. H. Williams, “Factors influencing the structure and function of the small intestine in the weaned pig: a review,” Livestock Production Science, vol. 51, no. 1–3, pp. 215–236, 1997. View at Publisher · View at Google Scholar · View at Scopus
  53. J. M. Mullin, M. C. Valenzano, J. J. Verrecchio, and R. Kothari, “Age- and diet-related increase in transepithelial colon permeability of Fischer 344 rats,” Digestive Diseases and Sciences, vol. 47, no. 10, pp. 2262–2270, 2002. View at Publisher · View at Google Scholar · View at Scopus
  54. M.-Y. Kim, L.-M. Chung, S.-J. Lee et al., “Comparison of free amino acid, carbohydrates concentrations in Korean edible and medicinal mushrooms,” Food Chemistry, vol. 113, no. 2, pp. 386–393, 2009. View at Publisher · View at Google Scholar · View at Scopus
  55. A. A. Soares, A. B. Sá-Nakanishi, A. L. Oliveira et al., “Effects of the aqueous estract of Agaricus blazei (Murrill) on the hepatic glycogen and lipids contents in rats submitted to injury by paracetamol,” in XLI Annual Meeting of SBBq, 2012.
  56. M. R. M. Natali, S. L. Molinari, L. C. Valentini, and M. H. de Miranda Neto, “Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration,” Biocell, vol. 29, no. 1, pp. 39–46, 2005. View at Google Scholar · View at Scopus
  57. P. R. Wade and T. Cowen, “Neurodegeneration: a key factor in the ageing gut,” Neurogastroenterology and Motility, vol. 16, no. 1, pp. 19–23, 2004. View at Publisher · View at Google Scholar · View at Scopus
  58. R. J. Phillips, B. S. Rhodes, and T. L. Powley, “Effects of age on sympathetic innervation of the myenteric plexus and gastrointestinal smooth muscle of Fischer 344 rats,” Anatomy and Embryology, vol. 211, no. 6, pp. 673–683, 2006. View at Publisher · View at Google Scholar · View at Scopus
  59. O. A. Gomes, R. R. de Souza, and E. A. Liberti, “A preliminary investigation of the effects of aging on the nerve cell number in the myenteric ganglia of the human colon,” Gerontology, vol. 43, no. 4, pp. 210–217, 1997. View at Publisher · View at Google Scholar · View at Scopus
  60. G. Gabella, “Fall in the number of myenteric neurons in aging guinea pigs,” Gastroenterology, vol. 96, no. 6, pp. 1487–1493, 1989. View at Google Scholar · View at Scopus
  61. T. Cowen, R. J. R. Johnson, V. Soubeyre, and R. M. Santer, “Restricted diet rescues rat enteric motor neurones from age related cell death,” Gut, vol. 47, no. 5, pp. 653–660, 2000. View at Publisher · View at Google Scholar · View at Scopus
  62. J. P. F. Schoffen, A. Soares, P. de Freitas, N. C. Buttow, and M. R. M. Natali, “Effects of a hypoproteic diet on myosin-V immunostained myenteric neurons and the proximal colon wall of aging rats,” Autonomic Neuroscience: Basic & Clinical, vol. 122, no. 1-2, pp. 77–83, 2005. View at Publisher · View at Google Scholar · View at Scopus
  63. K. Nakajima, I. Tooyama, O. Yasuhara, Y. Aimi, and H. Kimura, “Immunohistochemical demonstration of choline acetyltransferase of a peripheral type (pChAT) in the enteric nervous system of rats,” Journal of Chemical Neuroanatomy, vol. 18, no. 1-2, pp. 31–40, 2000. View at Publisher · View at Google Scholar · View at Scopus
  64. R. J. Phillips, S. L. Hargrave, B. S. Rhodes, D. A. Zopf, and T. L. Powley, “Quantification of neurons in the myenteric plexus: an evaluation of putative pan-neuronal markers,” Journal of Neuroscience Methods, vol. 133, no. 1-2, pp. 99–107, 2004. View at Publisher · View at Google Scholar · View at Scopus
  65. C. E. Bernard, S. J. Gibbons, P. J. Gomez-Pinilla et al., “Effect of age on the enteric nervous system of the human colon,” Neurogastroenterology & Motility, vol. 21, no. 7, p. 746-e4, 2009. View at Publisher · View at Google Scholar
  66. M. Sabbatini, P. Barili, E. Bronzetti, D. Zaccheo, and F. Amenta, “Age-related changes of glial fibrillary acidic protein immunoreactive astrocytes in the rat cerebellar cortex,” Mechanisms of Ageing and Development, vol. 108, no. 2, pp. 165–172, 1999. View at Publisher · View at Google Scholar · View at Scopus
  67. S. A. B. E. van Acker, M. N. J. L. Tromp, G. R. M. M. Haenen, W. J. F. van der Vijgh, and A. Bast, “Flavonoids as scavengers of nitric oxide radical,” Biochemical and Biophysical Research Communications, vol. 214, no. 3, pp. 755–759, 1995. View at Publisher · View at Google Scholar · View at Scopus
  68. I.-H. Wei, Y.-C. Wu, C.-Y. Wen, and J.-Y. Shieh, “Green tea polyphenol (-)-epigallocatechin gallate attenuates the neuronal NADPH-d/nNOS expression in the nodose ganglion of acute hypoxic rats,” Brain Research, vol. 999, no. 1, pp. 73–80, 2004. View at Publisher · View at Google Scholar · View at Scopus
  69. R. J. Phillips and T. L. Powley, “Innervation of the gastrointestinal tract: Patterns of aging,” Autonomic Neuroscience: Basic and Clinical, vol. 136, no. 1-2, pp. 1–19, 2007. View at Publisher · View at Google Scholar · View at Scopus
  70. G. L. Ferri, L. Probert, D. Cocchia, F. Michetti, P. J. Marangos, and J. M. Polak, “Evidence for the presence of S-100 protein in the glial component of the human enteric nervous system,” Nature, vol. 297, no. 5865, pp. 409–410, 1982. View at Publisher · View at Google Scholar · View at Scopus
  71. K. R. Jessen, R. Thorpe, and R. Mirsky, “Molecular identity, distribution and heterogeneity of glial fibrillary acidic protein: an immunoblotting and immunohistochemical study of Schwann cells, satellite cells, enteric glia and astrocytes,” Journal of Neurocytology, vol. 13, no. 2, pp. 187–200, 1984. View at Publisher · View at Google Scholar · View at Scopus
  72. V. Sharma, M. Mishra, S. Ghosh et al., “Modulation of interleukin-1β mediated inflammatory response in human astrocytes by flavonoids: implications in neuroprotection,” Brain Research Bulletin, vol. 73, no. 1-3, pp. 55–63, 2007. View at Publisher · View at Google Scholar · View at Scopus
  73. J. H. Hong, K. S. Youn, and Y. H. Choi, “Characteristics of crude protein-bound polysaccharide from Agaricus blazei Murill by extraction and precipitation conditions and its antitumor effect,” Korean Journal of Food Science and Technology, vol. 36, pp. 586–593, 2004. View at Google Scholar
  74. H. Abdo, P. Derkinderen, P. Gomes et al., “Enteric glial cells protect neurons from oxidative stress in part via reduced glutathione,” The FASEB Journal, vol. 24, no. 4, pp. 1082–1094, 2010. View at Publisher · View at Google Scholar · View at Scopus
  75. H. Muyderman, A. L. Wadey, M. Nilsson, and N. R. Sims, “Mitochondrial glutathione protects against cell death induced by oxidative and nitrative stress in astrocytes,” Journal of Neurochemistry, vol. 102, no. 4, pp. 1369–1382, 2007. View at Publisher · View at Google Scholar · View at Scopus
  76. P. de Freitas, M. R. M. Natali, R. V. F. Pereira, M. H. M. Neto, and J. N. Zanoni, “Myenteric neurons and intestinal mucosa of diabetic rats after ascorbic acid supplementation,” World Journal of Gastroenterology, vol. 14, no. 42, pp. 6518–6524, 2008. View at Publisher · View at Google Scholar · View at Scopus
  77. L. P. Roldi, R. V. F. Pereira, E. A. Tronchini et al., “Vitamin E (α-tocopherol) supplementation in diabetic rats: effects on the proximal colon,” BMC Gastroenterology, vol. 9, article 88, 2009. View at Publisher · View at Google Scholar · View at Scopus
  78. R. V. F. Pereira, E. A. Tronchini, C. M. Tashima, E. P. B. Alves, M. M. Lima, and J. N. Zanoni, “L-glutamine supplementation prevents myenteric neuron loss and has gliatrophic effects in the ileum of diabetic rats,” Digestive Diseases and Sciences, vol. 56, no. 12, pp. 3507–3516, 2011. View at Publisher · View at Google Scholar · View at Scopus
  79. G. G. P. da Silva, J. N. Zanoni, and N. C. Buttow, “Neuroprotective action of Ginkgo biloba on the enteric nervous system of diabetic rats,” World Journal of Gastroenterology, vol. 17, no. 7, pp. 898–905, 2011. View at Publisher · View at Google Scholar · View at Scopus
  80. C. R. P. Lopes, P. E. B. Ferreira, J. N. Zanoni, A. M. P. Alves, E. P. B. Alves, and N. C. Buttow, “Neuroprotective effect of quercetin on the duodenum enteric nervous system of streptozotocin-induced diabetic rats,” Digestive Diseases and Sciences, vol. 57, no. 12, pp. 3106–3115, 2012. View at Publisher · View at Google Scholar · View at Scopus
  81. L. C. Schneider, G. G. Perez, S. R. Banzi et al., “Evaluation of the effect of Ginkgo biloba extract (EGb 761) on the myenteric plexus of the small intestine of Wistar rats,” Journal of Gastroenterology, vol. 42, no. 8, pp. 624–630, 2007. View at Publisher · View at Google Scholar · View at Scopus
  82. G. G. Perez, L. C. L. Schneider, and N. C. Buttow, “Ginkgo biloba (EGb 761) extract: effects on the myenteric plexus of the large intestine in wistar rats,” Digestive Diseases and Sciences, vol. 54, no. 2, pp. 232–237, 2009. View at Publisher · View at Google Scholar · View at Scopus
  83. A. P. T. Veit and J. N. Zanoni, “Age-related changes in myosin-V myenteric neurons, CGRP and VIP immunoreactivity in the ileum of rats supplemented with ascorbic acid,” Histology and Histopathology, vol. 27, no. 1, pp. 123–132, 2012. View at Google Scholar · View at Scopus