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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 293768, 10 pages
http://dx.doi.org/10.1155/2015/293768
Research Article

Anti-Inflammatory Activity of Triterpenes Isolated from Protium paniculatum Oil-Resins

1Laboratório de Atividade Biológica, Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas (UFAM), Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil
2Instituto de Ciências Exatas, Departamento de Química, Universidade Federal do Amazonas, Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil
3Laboratório de Farmacologia, Departamento de Ciências Fisiológicas, Instituto de Ciências Biológicas, Universidade Federal do Amazonas, Avenida Gen. Rodrigo Otavio, No. 6200, 69077-000 Manaus, AM, Brazil

Received 17 August 2015; Revised 5 November 2015; Accepted 9 November 2015

Academic Editor: Ken Yasukawa

Copyright © 2015 Patrícia D. O. de Almeida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Protium is the main genus of the Burseraceae family and one of the most common genera in South America, with an important species called “breu.” Gum and oil-resins of this species are used as tonic and stimulant and for the treatment of ulcers and inflammation. The present study aims to isolate and investigate the anti-inflammatory activity of triterpene compounds isolated from oil-resin of Protium paniculatum. The pentacyclic triterpenes α,β-amyrin, acetylated α,β-amyrin, α,β-amyrone, and brein/maniladiol did not alter the viability of murine J774 macrophages (IC50 > 20 µg/mL), with the exception of mixture of brein/maniladiol which showed moderate cytotoxic activity. Also it was observed that compounds at 10 µg/mL inhibited more than 80% of production of NO, although only α,β-amyrin was able to inhibit the production of TNF-α (). The compounds inhibited the production of IL-6 and induced the production of IL-10 in murine J774 macrophages stimulated by LPS. α,β-Amyrone inhibited the expression of COX-2 and also inhibited the formation of paw or ear edema in rats and mice, having a quick and immediate effect. This study may provide the basis for future investigations on the therapeutic role of α,β-amyrone in treating inflammation.