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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 296086, 10 pages
Research Article

Antiviral Action of Hydromethanolic Extract of Geopropolis from Scaptotrigona postica against Antiherpes Simplex Virus (HSV-1)

1Instituto Butantan, Avenida Vital Brasil, No. 1500, Butantã, 05503-900 São Paulo, SP, Brazil
2Instituto Adolfo Lutz, Avenida Dr. Arnaldo 355, 01246-900 São Paulo, SP, Brazil
3CEBRID, Departamento de Medicina Preventiva, Universidade Federal de São Paulo, Rua Botucatu 740, Vila Clementino, 04023-900 São Paulo, SP, Brazil

Received 26 November 2014; Revised 13 February 2015; Accepted 15 February 2015

Academic Editor: Andreas Sandner-Kiesling

Copyright © 2015 Guilherme Rabelo Coelho et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The studies on chemical composition and biological activity of propolis had focused mainly on species Apis mellifera L. (Hymenoptera: Apidae). There are few studies about the uncommon propolis collected by stingless bees of the Meliponini tribe known as geopropolis. The geopropolis from Scaptotrigona postica was collected in the region of Barra do Corda, Maranhão state, Brazil. The chemical analysis of hydromethanolic extract of this geopropolis (HMG) was carried out through HPLC-DAD-ESI-MS/MS and the main constituents found were pyrrolizidine alkaloids and C-glycosyl flavones. The presence of alkaloids in extracts of propolis is detected for the first time in this sample. The antiviral activity of HMG was evaluated through viral DNA quantification experiments and electron microscopy experiments. Quantification of viral DNA from herpes virus showed reduction of about 98% in all conditions and concentration tested of the HMG extract. The results obtained were corroborated by transmission electron microscopy, in which the images did not show particle or viral replication complex. The antiviral activity of C-glycosyl flavones was reported for a variety of viruses, being observed at different points in the viral replication. This work is the first report about the antiviral activity of geopropolis from Scaptotrigona postica, in vitro, against antiherpes simplex virus (HSV).