Huqi San-Evoked Rat Colonic Anion Secretion through Increasing CFTR Expression

Figure 1

Real-time RT-PCR analysis of mRNA expression of CFTR and α-ENaC in rat liver, distal colon, and pancreas. (a) Continuous monitoring of fluorescence emission during cycle PCR of template DNA from strains tested. The number of samples for liver, pancreas, and distal colon is 35 and 40, respectively. The error bars represent standard error. (b) Representative different tissues experiment by melting analysis based on real-time PCR using SYBR Green I. The melting point peaks were clearly separated among liver, pancreas, and distal colon using SYBR Green I at 2.5 mM MgCl₂. Real time RT-PCR results with products of CFTR found in rat liver, pancreas, and distal colon quantitative analysis of CFTR expression pretreatment with RP, RA, and HQS, respectively, and GAPDH (internal marker) ratio shown. RA and HQS could enhance the CFTR expression in rat liver and distal colon but not in pancreas ((c)–(e)). Quantitative analysis of α-ENaC expression pretreatment with RP, RA, and HQS, respectively, and GAPDH (internal marker) ratio were shown. There is no statistically significant change in liver, pancreas, or distal colon ((f)–(h)). Data analysis was used with one-way analysis of variance followed by Dunnett’s multiple comparison. Values are represented as means ± SEM; , .

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