Huqi San-Evoked Rat Colonic Anion Secretion through Increasing CFTR Expression

Figure 2

Western blot confirmed the expression of CFTR and α-ENaC in rat liver, distal colon, and pancreas. Densitometric analysis of CFTR and α-ENaC protein levels (normalized to GAPDH) in rat liver. HQS (1.0 mg/mL) can significantly increase the expression of CFTR in liver (a). Densitometric analysis of CFTR and α-ENaC protein levels (normalized to GAPDH) in rat distal colon. HQS (1.0 mg/mL) and RA can significantly increase the expression of CFTR in colon (b). Densitometric analysis of CFTR and α-ENaC protein levels (normalized to GAPDH) in rat pancreas. HQS (1.0 mg/mL) can significantly increase the expression of CFTR in pancreas (c). α-ENaC has no conspicuous changes across different groups ((a)–(c)). (d) Effects of Cl⁻ replacement and transporter inhibitors on the HQS . The bar graph illustrates the effects of removing extracellular Cl⁻ on the HQS and transepithelial resistance (e). Representative traces of HQS-evoked Cl⁻ flux in the absence and presence of Cl⁻. Arrows indicate the time of the response to HQS (1.0 mg/mL) added basal in normal and Cl⁻-free K-H solutions (f). The bar graph illustrates the effects of apical addition of amiloride (10 μmol/L) on the . HQS (1.0 mg/mL) added basal in normal and Na⁺ channel blocker, amiloride (10 μmol/L) (g). The bar graph illustrates of the amiloride on the transepithelial resistance of HQS (1.0 mg/mL) (h). Representative traces of HQS-evoked in the absence and presence of amiloride (100 μM). Arrowheads indicate the time of HQS addition (i). The bar graph illustrates the application of glibenclamide (1 mmol/L, apical), an inhibitor of the CFTR transporter or bumetanide (100 μmol/L, basal), and the Na⁺-K⁺-2Cl⁻ cotransporter on HQS-induced comparison of HQS- (1.0 mg/mL) induced (j). Representative traces of recording with arrows indicating the time for the apical application of glibenclamide (1 mm/L) or basolateral application of bumetanide (100 μmol/L), respectively, and addition of HQS (1.0 mg/mL) (k). Data analysis was used with one-way analysis of variance followed by Dunnett’s multiple comparison. Values are represented as mean ± SEM; , .

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