Comparisons of Ethanol Extracts of Chinese Propolis (Poplar Type) and Poplar Gums Based on the Antioxidant Activities and Molecular Mechanism

Figure 5

EECP and EEPG activate the expression of antioxidant genes at protein levels in RAW264.7 cells. ((a)–(d)) RAW264.7 cells were cultured in the presence or absence of EECP and EEPG at the indicated lengths time to detect the time-dependent inductions of the HO-1, γ-GCLM, and TrxR1 protein. ((e)–(g)) The activation effects of dose-dependent induction of the HO-1, γ-GCLM, and TrxR1 protein. RAW264.7 cells were incubated with indicated concentrations of EECP and EEPG for 9 h. All the expressions of the protein were detected by western blot, and the expression of β-tubulin protein was used as an internal control. Each value of (b)–(d), (f), and (g) represented the ratio of the density of each stripe of antioxidant genes and the density of each strip of β-tubulin protein, respectively. And the values were calculated by image J.

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