Purification and Characterization of Asparaginase from Phaseolus vulgaris Seeds

Figure 2

Gel filtration of asparaginase I from DEAE-Sepharose fraction on Sephacryl S-200 column (90 × 1.6 cm i.d.). The column was equilibrated with 20 mM Tris-HCl buffer, pH 8.0 at a flow rate of 30 mL/h and 3 mL fractions.