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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 319240, 10 pages
Research Article

Antifungal and Antibacterial Metabolites from a French Poplar Type Propolis

1EA 921 SONAS/SFR 4207 QUASAV, Université d’Angers, 16 boulevard Daviers, 49045 Angers Cedex 01, France
2Laboratoire de Bactériologie-Hygiène, Centre Hospitalier Universitaire, 4 rue Larrey, 49933 Angers Cedex 09, France
3Groupe d’Etude des Interactions Hôte Pathogène (GEIHP), Université d’Angers, 4 rue Larrey, 49933 Angers Cedex, France
4Ballot-Flurin Apiculteurs-Abeilles Santé, 75 place Lagardère, 65700 Maubourguet, France

Received 2 February 2015; Revised 3 March 2015; Accepted 4 March 2015

Academic Editor: Veronique Seidel

Copyright © 2015 Séverine Boisard et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


During this study, the in vitro antifungal and antibacterial activities of different extracts (aqueous and organic) obtained from a French propolis batch were evaluated. Antifungal activity was evaluated by broth microdilution on three pathogenic strains: Candida albicans, C. glabrata, and Aspergillus fumigatus. Antibacterial activity was assayed using agar dilution method on 36 Gram-negative and Gram-positive strains including Staphylococcus aureus. Organic extracts showed a significant antifungal activity against C. albicans and C. glabrata (MIC80 between 16 and 31 µg/mL) but only a weak activity towards A. fumigatus (MIC80 = 250 µg/mL). DCM based extracts exhibited a selective Gram-positive antibacterial activity, especially against S. aureus (SA) and several of its methicillin-resistant (MRSA) and methicillin-susceptible (MSSA) strains (MIC100 30–97 µg/mL). A new and active derivative of catechin was also identified whereas a synergistic antimicrobial effect was noticed during this study.