Research Article

Ethanol Extract of Perilla frutescens Suppresses Allergen-Specific Th2 Responses and Alleviates Airway Inflammation and Hyperreactivity in Ovalbumin-Sensitized Murine Model of Asthma

Figure 1

Schematic diagram of the experimental protocol. Eight-week-old female BALB/c mice were sensitized intraperitoneally (i.p.) by OVA for three times (10, 30, and 30 μg/mouse) on days 0, 14, and 28. OVA-sensitized mice were challenged with 5% aerosolized OVA three times on days 49, 56, and 62. After 3 weeks of supplementation, mice were sacrificed and the BALF, serum, and splenocytes of mice were collected for further analysis.