Ethanol Extract of Perilla frutescens Suppresses Allergen-Specific Th2 Responses and Alleviates Airway Inflammation and Hyperreactivity in Ovalbumin-Sensitized Murine Model of Asthma

Figure 2

The Th2 cytokines produced by OVA-stimulated splenocytes from OVA-sensitized/challenged BALB/c mice supplemented with different extracts of Perilla frutescens. OVA-sensitized mice were fed with water (PfWL or PfWH) or ethanol (PfEL or PfEH) extracts of Perilla frutescens for 3 weeks, respectively. Splenocytes were isolated from OVA-sensitized/challenged mice and stimulated with OVA (100 μg/mL for IL-4, 50 μg/mL for IL-5 and IL-13) for 48 hours. The productions of Th2 cytokines in supernatant were determined by ELISA. Values represent mean ± SD, = 7~8 for each OVA-sensitized group, and for the PBS group as negative control. Statistical analysis was performed with Student’s -test, , compared with the control group.