Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

Figure 11

Western blot analysis of Fas and mitochondrial/cytosolic Cytochrome-c protein expression levels in HL-7702 cells exposed to different doses of rhein. (a) Representative results of western blotting analysis. β-actin served as a loading control. (b) The quantification of the immunoblots was analyzed by densitometric scanning. Band densities were digitized and relative band intensities of target proteins were normalized against the β-actin levels. Data were expressed as mean ± SD from three different experiments, , , significantly different from control values.

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