Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

Figure 5

(a) Triglyceride (TG) and total cholesterol (TC) studies. Rhein induced in vitro triglyceride and total cholesterol upregulation in a dose-dependent manner. HL-7702 cells were incubated with different concentration of rhein for 12 h and then processed for TG and TC colorimetric assay. Analysis of TG and TC elevation was made relative to untreated control cells (100%). Data are expressed as mean ± SD from three independent experiments. (b) Quantitative analysis of HGM-CoAR, ACoAC, and GPAT mRNA expression levels in HL-7702 cells exposed to different doses of Rhein. GAPDH was used as internal positive control standard. The relative expression of target genes was calculated using method. Data are expressed as mean ± SD from three different experiments. ( versus control and versus control).

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