Rhein Elicits In Vitro Cytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

Table 1

Sequences of the primers used in real-time PCR.

Gene description	Primer	Sequence (5′→3′)	Length (bp)
GAPDH	GAPDH-F	CAGGAGGCATTGCTGATGAT	20
	GAPDH-R	GAAGGCTGGGGCTCATTT	18
HGM-CoAR	HGM-CoAR-F	AGCCTGAATAGCCCGACAG	19
	HGM-CoAR-R	CATCCTCCACAAGACAATGC	20
GPAT	GPAT-F	TGAACAACTGGGCAAACCTAA	21
	GPAT-R	AAATCCACTCGGACACAACC	20
ACoAC	ACoAC-F	CTCTTGACCCTGGCTGTGTA	20
	ACoAC-R	GATGGAGTTTCTCGCCTCTG	20
p53	p53-F	GCCATCTACAAGCAGTCACAG	21
	p53-R	ATTTCCTTCCACTCGGATAAGA	22
PUMA	PUMA-F	GAAGAGCAAATGAGCCAAAC	20
	PUMA-R	CAGAGCACAGGATTCACAGTCT	22
Fas	Fas-F	ACACTCACCAGCAACACCAAGT	22
	Fas-R	CCTTTCTCTTCACCCAAACAAT	22
Cyt-c	Cyt-c-F	TACTCTTACACAGCCGCCAATA	22
	Cyt-c-R	AGTCTGCCCTTTCTTCCTTCTT	22
Apaf-1	Apaf-1-F	GTGAAGTGTTGTTCGTGGTCTG	22
	Apaf-1-R	CGTGTGGATTTCTCCCAATAG	21
Casp-8	Casp-8-F	ATGTTGGAGGAAAGCAATCTGT	22
	Casp-8-R	CCTGCCTGGTGTCTGAAGTT	20
Casp-9	Casp-9-F	ACTAACAGGCAAGCAGCAAAGT	22
	Casp-9-R	ACATCACCAAATCCTCCAGAAC	22
Casp-3	Casp-3-F	AGCAATAAATGAATGGGCTGAG	22
	Casp-3-R	GTATGGAGAAATGGGCTGTAGG	22