An In Vitro System Comprising Immortalized Hypothalamic Neuronal Cells (GT1–7 Cells) for Evaluation of the Neuroendocrine Effects of Essential Oils

Figure 4

The effect of essential oils on TMX-induced GT1–7 cell cytotoxicity. (a) GT1–7 cells were treated with 0.5 μM TMX without (aroma (—) in graph) or with 25 ppm of various essential oils. After 24 h, the viability was analyzed using the WST-1 method. The white bar shows the viability of GT1–7 cells treated with essential oil alone. Data are presented as the mean ± SEM (). (b) The dose dependency of the effects of essential oils on TMX-induced GT1–7 cell cytotoxicity. 10–25 ppm of essential oils was treated with 0.5 μM TMX. After 24 h, the viability was analyzed using the WST-1 method. Data are presented as the mean ± SEM (). , versus the aroma (—) group.

(a) With TMX

(b) Dose response