Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice

Figure 2

Moderate dose of XBJ (5 mg/mL) enhanced the expression of M2 phenotype markers of mouse peritoneal macrophages. Peritoneal macrophages of mice were, respectively, treated with 0 mg/mL, 1 mg/mL, 5 mg/mL, 10 mg/mL, 50 mg/mL, and 100 mg/mL XBJ in the presence of 100 ng/mL LPS for 6 h, 24 h, or 48 h. Cytokines levels including TNF-α, IL-6, and IL-10 in the medium were determined by ELISA (a). F4/80, CD11c, and CD206 expressions were determined by flow cytometry. The group of F4/80⁺CD11c⁺CD206⁻  was classified as M1 and F4/80⁺CD11c⁻CD206⁺ as M2 (b). The treated macrophages were lysed and levels of Arg1, Ym1, and Fizz1 were measured by western blot (c). Data represented the mean ± SEM of independent experiment in triplicate. , , and versus control group.

(a)

(b)

(c)