Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 4

Research Article

Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice

Figure 4

Treatment with JAK1 and STAT6 inhibitors could markedly diminish the polarization of macrophages by XBJ. Mouse peritoneal macrophages were, respectively, treated with 0 mg/mL or 5 mg/mL XBJ in the presence of 100 ng/mL LPS with or without JAK1 inhibitor or STAT6 inhibitor (leflunomide) for 24 h. TNF-α, IL-6, and IL-10 levels in the medium were assessed by ELISA (a). F4/80, CD11c, and CD206 expressions were analyzed by flow cytometry. The group of F4/80+CD11c+CD206  was classified as M1 and F4/80+CD11cCD206+ as M2 (b). Data represented the mean ± SEM of independent experiment in triplicate. versus control group.

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