Xuebijing Injection Promotes M2 Polarization of Macrophages and Improves Survival Rate in Septic Mice

Figure 5

XBJ promoted the polarization of mouse peritoneal macrophages in septic mice in vivo. Balb/c mice were subjected to severe sepsis induced by CLP, and XBJ (4 mg/kg) was intramuscularly administered at 0.5 h and 12 h after CLP. Serum levels of TNF-α, IL-6, and IL-10 were determined by ELISA at 24 h after CLP (a). Peritoneal macrophages were collected and F4/80, CD11c, and CD206 expressions were analyzed by flow cytometry at 24 h after CLP. The group of F4/80⁺CD11c⁺CD206⁻  was classified as M1 and F4/80⁺CD11c⁻CD206⁺ as M2 (b). Data represented the mean ± SEM of three independent experiments. versus control group.

(a)

(b)