Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 3

Research Article

Naringin Attenuates Autophagic Stress and Neuroinflammation in Kainic Acid-Treated Hippocampus In Vivo

Figure 3

Reduction of autophagic stress by naringin treatment in the KA-treated hippocampus. Mice received twice intraperitoneal injection of naringin at 1 day and 1 h before KA treatment, and mice were sacrificed 1 day after KA treatment. Western blot analysis shows the bands of LC3 (a). There is no significant difference to the level of LC3 II by NAR(80) compared to CON. However, NAR(80) significantly reduced the level of KA-induced LC3 II. Quantitative data are calculated from the density of LC3 II bands normalized to the density of β-actin bands for each sample. and , significantly different from CON and KA alone, respectively (one-way ANOVA and Tukey post hoc test, for each group). Representative pictures of double immunofluorescence staining show LC3B-positive punctate signals within NeuN-stained neurons in the KA-treated hippocampal CA1 area (b). Naringin treatment attenuates LC3B punctate signals in the hippocampal CA1 neurons treated by KA. Scale bar: 20 μm and 10 μm, respectively.

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19.