Effect of Aqueous Extract from Morinda officinalis F. C. How on Microwave-Induced Hypothalamic-Pituitary-Testis Axis Impairment in Male Sprague-Dawley Rats

Figure 6

Representative coronal images of the arcuate nucleus of the hypothalamus showing immunohistochemical double labeling for GnRH/DAPI. Photomicrographs of DAPI ((a)–(d)) and GnRH ((e)–(h)) and merged images of DAPI and GnRH ((i)–(l)). The yellow arrow represents positive GnRH expression. Treatment with water layer fraction of aqueous extract 40 g/kg significantly decreased the GnRH fluorescence intensity of the hypothalamus compared with the microwave exposure group. GnRH = gonadotrophin-releasing hormone; TV = third ventricle. DAPI = 4,6-diamidino-2-phenylindole. Scale bar = 250 μm.