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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 361607, 9 pages
http://dx.doi.org/10.1155/2015/361607
Research Article

Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes

1School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China
2Institute of Rheumatology and Immunology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China
3Nephrology Department, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China

Received 3 January 2015; Revised 15 March 2015; Accepted 16 March 2015

Academic Editor: Klaus Heese

Copyright © 2015 Wan-Tai Dang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A large number of studies have shown that cysteinyl aspartate specific protease-1 (CASP1) played an important role in the inflammatory response of primary gout, but the decreased expression of different CASP1 transcript variant could inhibit the activation of IL-1. Our study mainly analyzed the expression level and function of CASP1 gene transcript variant mRNA in peripheral blood mononuclear cells of patients with gout in different TCM syndromes. The expression of CASP1 gene transcript variant and IL-1 mRNA in PBMCs were detected in patients with PG [acute phase (AP: 44 cases); nonacute phase (NAP: 52 cases)] and healthy controls (HC: 30 cases) by reverse transcription-polymerase chain reaction and/or real-time quantitative polymerase chain reaction. The expressions of plasma IL-1 in patients with PG and HC were detected by enzyme-linked immunosorbent assay. Dysregulated expression of the CASP1 gene and its transcript variant, plasma proinflammatory cytokines in all patients with primary gout in different TCM syndromes, correlation analysis showed that there was negative correlation between the expression of CASP1-gamma gene transcript variant mRNA and IL-1 protein in APPG group. The study suggested that CASP1 gene and its transcript variant may play a critical role in the inflammatory response of patients with PG in different phases and TCM syndromes.