Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes

Figure 2

The mRNA expression of CASP1 gene and its transcript variant in PBMCs of patients with PG in different phases. Notes: HC: health control, AP: acute phase of primary gout, NAP: nonacute phase of primary gout; (a) CASP1 gene primers were amplified to one fragment (96 bp); (b) common primers of CASP1 gene transcript variants 6, 7, beta, alpha, and gamma were amplified to six fragments: 1774 bp was transcript variant 6, 1711 bp was transcript variant 7, 1086 bp was transcript variant beta, 1149 bp was transcript variant alpha, 833 bp was transcript variant gamma, and x was an unknown stripe. Delta and epsilon stripe were not observed in designed primers of our research, and we will carry on the design and experiment through different methods. (c) The RNA quality electropherogram of PBMCs. ; .

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