Research Article
Expression of Caspase-1 Gene Transcript Variant mRNA in Peripheral Blood Mononuclear Cells of Patients with Primary Gout in Different TCM Syndromes
Table 1
Primer sequences of CASP1 gene and its transcript variant.
| | Gene (transcript variant) name | Upstream | Downstream | Genetic fragment size |
| | CASP1 | 5′-CGCAGATGCCCACCACT-3′ | 5′-TGCCCACAGACATTCATACAG-3′ | 96 bp | | CASP1-6 (NM_001257118.2) | 5′-TACAGTTATGGATAAGACCCGAGC-3′ | 5′-GCAGACATAATTCCAAAAACCTTTA-3′ | 1774 bp | | CASP1-7 (NM_001257119.2) | 5′-TACAGTTATGGATAAGACCCGAGC-3′ | 5′-GCAGACATAATTCCAAAAACCTTTA-3′ | 1711 bp | | CASP1-alpha (NM_033292.3) | 5′-TACAGTTATGGATAAGACCCGAGC-3′ | 5′-GCAGACATAATTCCAAAAACCTTTA-3′ | 1149 bp | | CASP1-beta (NM_001223.4) | 5′-TACAGTTATGGATAAGACCCGAGC-3′ | 5′-GCAGACATAATTCCAAAAACCTTTA-3′ | 1086 bp | | CASP1-gamma (NM_033293.3) | 5′-TACAGTTATGGATAAGACCCGAGC-3′ | 5′-GCAGACATAATTCCAAAAACCTTTA-3′ | 833 bp | | IL-1 | 5′-ACAGATGAAGTGCTCCTTCCA-3′ | 5′-GTCGGAGATTCGTAGCTGGAT-3′ | 73 bp | | -actin | 5′-GAGCTACGAGCTGCCTGACG-3′ | 5′-GTAGTTTCGTGGATGCCACAG-3′ | 120 bp |
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