Luteolin Exerts Cardioprotective Effects through Improving Sarcoplasmic Reticulum Ca²⁺-ATPase Activity in Rats during Ischemia/Reperfusion In Vivo

Table 1

Surgical protocol, group distribution, and observation time of three experiment stages.

Experiment; stage	Groups	Observation time ( at each time point in each group)	Test items
First step	Sham; I/R; Lut + I/R; Lut + LY + I/R	Ischemia for 30 min followed by reperfusion for 1 d	Myocardial infarct size; LDH release; Apoptosis-related proteins
Second step	Sham; I/R; Lut + I/R	Ischemia for 30 min followed by reperfusion for 0, 30, 60, and 120 min; 6, 12 h; 1, 3, 5, and 7 d and ischemia for 60 min followed by reperfusion for 30 min	Protein expression and activity of SERCA2a
Third step	Sham; I/R; Lut + I/R; Lut + LY + I/R	Ischemia for 30 min followed by reperfusion for 120 min	Protein expressions of Akt, p-Akt 308, and p-Akt 473; Protein expression and activity of SERCA2a