Arctigenin from Fructus Arctii (Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers

Figure 2

Fructus Arctii extract increased TEER value and decreased OVA influx in Caco-2 cells. The monolayers of Caco-2 cells were preincubated with HBSS for 30 min at 37°C in a CO₂ incubator to stabilize the cell monolayers. The cell monolayers were treated with each sample and bile salts for 60 min, and OVA was added for 3 h at 37°C. (a) Changes of TEER were measured by treating the cell monolayers with nine extracts (400 μg/mL) and (b) Fructus Arctii extract (FAE) at various doses (100, 200, and 400 μg/mL) for 3 h. (c and d) Changes in TEER value and OVA influx induced by FAE were detected by ELISA. Each value is presented as mean ± SD (). Bars are significantly different from the control at and . Data were analyzed using ANOVA followed by Duncan’s multiple-range test.

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