Arctigenin from Fructus Arctii (Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers

Figure 4

Arctigenin, arctiin, from Fructus Arctii extract enhanced intestinal barrier function in Caco-2 cells. (a and b) To identify active components in Fructus Arctii extract, HPLC analysis was carried out with a Jasco PU-2080 plus liquid chromatography system equipped with multiwavelength detector. Ethyl acetate and chloroform fractions were injected (1 μg/mL each) by autoinjector, and an ODS C18 column (250 × 4.6 mm) was used to separate compounds at 40°C in column adapter. Solvents used were a mixture of acetonitrile (eluent A) and water (eluent B) in gradient mode (eluent A: 20 to 40% in 35 min), and the flow rate was 1 mL/min. UV wavelength for detection was 280 nm. (c–f) The effects of arctiin and arctigenin on TEER and OVA permeability were examined in Caco-2 cells. Each value is presented as mean ± SD (). Bars are significantly different from the control at and . Data were analyzed using ANOVA followed by Duncan’s multiple-range test.

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