Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

Figure 5

EVA significantly reduces hemoglobin content in VEGF induced angiogenesis in Matrigel plugs of mice. Six-week-old C57BL/6 mice were given EVA (300 μg/kg) extracts via oral administration for 7 days before growth factor reduced Matrigel with VEGF (25 ng) and heparin (5 U) was subcutaneously injected into right flank of C57BL/6. Ten days later, Matrigel plugs were removed. (a) The amount of hemoglobin (Hb) was measured using the hemoglobin assay kit. ((A), (B), and (C) represent negative control, positive control, and EVA-treated group, resp.) (b) Bar graph represents the quantification of Hb concentration from each of groups. Data are presented as means ± SD. versus positive control group.

(a)

(b)