Antiangiogenic Effect of Ethanol Extract of Vigna angularis via Inhibition of Phosphorylation of VEGFR2, Erk, and Akt

Figure 6

EVA effectively attenuates the phosphorylation of VEGFR2, Erk, and Akt in VEGF-treated HUVECs. Cells were treated with 10 or 20 μg/mL of EVA in the presence of VEGF for 20 h and lysed in RIPA buffer. (a) The cell lysates were prepared and subjected to Western blotting for p-VEGFR2, VEGFR2, p-Akt, Akt, p-Erk, Erk, and β-actin. (b) Bar graphs represent fold change between actin and p-VEGFR2, p-Akt, or p-Erk groups.

(a)

(b)