Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Fig 1

Research Article

Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on the Ovariectomized Mice

Figure 1

HD suppresses osteoclastogenesis. (a) BMMs prepared from bone marrow cells were cultured for 4 days with RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the indicated concentrations of HD. Cells were fixed in 3.7% formalin, permeabilized in 0.1% Triton X-100, and stained for TRAP, an enzyme marker of osteoclasts. (b) TRAP-positive multinuclear cells with three or more nuclei were counted as osteoclasts. (c) Effect of HD on the viability on BMMs was evaluated by CCK-8 assay. ***.