Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on the Ovariectomized Mice
KOGHD suppresses osteoclastogenesis. (a) BMMs prepared from bone marrow cells were cultured for 4 days with RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the indicated concentrations of KOG and KOGHD. Cells were fixed in 3.7% formalin, permeabilized in 0.1% Triton X-100, and stained for TRAP, an enzyme marker of osteoclasts. (b) TRAP-positive multinuclear cells with three or more nuclei were counted as osteoclasts. (c) Effect of KOG-HD on the viability on BMMs was evaluated by CCK-8 assay. ***.