Evidence-Based Complementary and Alternative Medicine / 2015 / Article / Tab 2

Research Article

Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A2A Receptors

Table 2

Adenosine receptor A2A functional assay parameters.

Adenosine A2A adenylyl cyclase

SourceHuman recombinant HEK-293 cells
Control 0.1 M NECA
Vehicle0.40% DMSO
Incubation time/temp.10 minutes at 37°C
Incubation bufferModified Hank’s balanced salt solution (HBSS) pH 7.4
Quantitation method HTRF quantitation of cAMP accumulation
Significant criteria for agonist≥50% increase in cAMP relative to NECA response
Significant criteria for antagonist≥50% inhibition of NECA-induced cAMP increase

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