Effects of a Proprietary Standardized Orthosiphon stamineus Ethanolic Leaf Extract on Enhancing Memory in Sprague Dawley Rats Possibly via Blockade of Adenosine A_2A Receptors

Table 2

Adenosine receptor A_2A functional assay parameters.

Adenosine A2A adenylyl cyclase
Source	Human recombinant HEK-293 cells
Control	0.1 M NECA
Vehicle	0.40% DMSO
Incubation time/temp.	10 minutes at 37°C
Incubation buffer	Modified Hank’s balanced salt solution (HBSS) pH 7.4
Quantitation method	HTRF quantitation of cAMP accumulation
Significant criteria for agonist	≥50% increase in cAMP relative to NECA response
Significant criteria for antagonist	≥50% inhibition of NECA-induced cAMP increase