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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 376029, 10 pages
Research Article

Alterations in Salivary Proteome following Single Twenty-Minute Session of Yogic Breathing

1Department of Radiation Oncology, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA
2Division of Nephrology, Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
3Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, SC 29425, USA

Received 13 August 2014; Revised 5 December 2014; Accepted 22 December 2014

Academic Editor: Senthamil R. Selvan

Copyright © 2015 Sundaravadivel Balasubramanian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Yogic breathing (YB) has been suggested to reduce stress and blood pressure and increase cognitive processes. However, alterations after YB at the molecular level are not well established. Twenty healthy volunteers were randomized into two groups ( per group): YB or attention controls (AC). The YB group performed two YB exercises, each for ten minutes, for a total of twenty minutes in a single session. AC group read a text of their choice for 20 minutes. Saliva was collected at baseline and at 5, 10, 15, and 20 minutes. Using Mass Spectrometry (MS), we initially found that 22 proteins were differentially expressed and then validated deleted in malignant brain tumor-1 (DMBT1) and Ig lambda-2 chain C region (IGLC2) using Western Blotting. DMBT1 was elevated in 7 of YB group by 10-fold and 11-fold at 10 and 15 minutes, respectively, whereas it was undetectable in the time-matched AC group (). There was a significant interaction between groups and time assessed by two-way ANOVA (). IGLC2 also showed a significant increase in YB group as measured by Western Blotting. These data are the first to demonstrate the feasibility of stimulating and detecting salivary protein biomarkers in response to an acute Yoga exercise. This trial is registered with NCT02108769.