Safety Evaluation of Chinese Medicine Injections with a Cell Imaging-Based Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity

Figure 2

Acetaminophen (AAP), doxorubicin hydrochloride (DOX), and FCCP-generated dose-response curves deduced from cell number (a), mitochondrial mass (b), nuclear area (c), mitochondrial membrane potential (d), and plasma membrane permeability (e). Exposed to acetaminophen (1 μM, 10 μM, 100 μM, 1 mM, 10 mM, and 100 mM), exposed to doxorubicin hydrochloride (1 nM, 10 nM, 100 nM, 1 μM, 10 μM, and 100 μM), and exposed to FCCP (32 nM, 160 nM, 800 nM, 4 μM, 20 μM, and 100 μM), HepG2 cells are studied. Data is expressed as mean ± SEM, .

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