Safety Evaluation of Chinese Medicine Injections with a Cell Imaging-Based Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity

Figure 3

Representative images of the HCA analysis of HepG2 cells for the evaluation of nuclei number and morphology stained by 4 dyes in control (a, f, k, p, and u), Danhong injection (DHI) 100-fold dilution (b, g, l, q, and v), Xiangdan injection (XDI) 100-fold dilution (c, h, m, r, and w), Mailuoning injection (MLNI) 100-fold dilution (d, i, n, s, anad x), and Fufangkushen injection (FFKSI) 100-fold dilution (e, j, o, t, and y) groups. The cell number (CN) decreased by XDI 100-fold dilution and FFKSI 100-fold dilution (c, e). The intensity of Rhodamine 123 (green) was lower in FFKSI-treated group than control group indicating that the MMP decreased (o). Note: IC 50 of cell parameters associated with cell number (CN) was IC 50₁ and IC 50 of cell parameters associated with nuclear area (NA) was IC 50₂.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)

(r)

(s)

(t)

(u)

(v)

(w)

(x)

(y)