Safety Evaluation of Chinese Medicine Injections with a Cell Imaging-Based Multiparametric Assay Revealed a Critical Involvement of Mitochondrial Function in Hepatotoxicity

Figure 4

Effects of Danhong injection (DHI), Xiangdan injection (XDI), Mailuoning injection (MLNI), and Fufangkushen injection (FFKSI) on cell number (a), nuclear area (b), mitochondrial mass (c), mitochondrial membrane potential (d), and plasma membrane permeability (e). HepG2 cells were treated with DHI, XDI, MLNI, and FFKSI. FFKSI (100-fold dilution) and XDI (100-fold dilution) caused deviations from the control group with a significant decrease of the cell number (a). A significant increase of MS was induced by XDI (100-fold dilution) (b). All FFKSI group (100-, 300-, 500-, 800-, and 1000-fold dilution) induced a decrease of MMP (d). Data is expressed as mean ± SEM. , , .

(a)

(b)

(c)

(d)

(e)