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Evidence-Based Complementary and Alternative Medicine
Volume 2015 (2015), Article ID 391767, 9 pages
Research Article

Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice

1Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan
2Probiotics Research Center, National Yang-Ming University, Taipei 11221, Taiwan
3School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan
4Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan
5Division of Animal Technology, Animal Technology Laboratories, Agricultural Technology Research Institute, Zhunan Township, Miaoli County 35053, Taiwan

Received 23 September 2014; Revised 14 January 2015; Accepted 15 January 2015

Academic Editor: Jian-Guo Chen

Copyright © 2015 Chien-Chen Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Recent studies have demonstrated beneficial effects of specific probiotics on alleviating obesity-related disorders. Here we aimed to identify probiotics with potential antiobesity activity among 88 lactic acid bacterial strains via in vitro screening assays, and a Lactobacillus plantarum strain K21 was found to harbor abilities required for hydrolyzing bile salt, reducing cholesterol, and inhibiting the accumulation of lipid in 3T3-L1 preadipocytes. Furthermore, effects of K21 on diet-induced obese (DIO) mice were examined. Male C57Bl/6J mice received a normal diet, high-fat diet (HFD), or HFD with K21 administration (109 CFU in 0.2 mL PBS/day) for eight weeks. Supplementation of K21, but not placebo, appeared to alleviate body weight gain and epididymal fat mass accumulation, reduce plasma leptin levels, decrease cholesterol and triglyceride levels, and mitigate liver damage in DIO mice. Moreover, the hepatic expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) related to adipogenesis was significantly downregulated in DIO mice by K21 intervention. We also found that K21 supplementation strengthens intestinal permeability and modulates the amount of Lactobacillus spp., Bifidobacterium spp., and Clostridium perfringens in the cecal contents of DIO mice. In conclusion, our results suggest that dietary intake of K21 protects against the onset of HFD-induced obesity through multiple mechanisms of action.