Electroacupuncture Suppressed Neuronal Apoptosis and Improved Cognitive Impairment in the AD Model Rats Possibly via Downregulation of Notch Signaling Pathway

Figure 2

EA prevented the neuronal apoptosis induced by Aβ in hippocampus tissue through regulating the expression of Bcl-2 and Bax. (a) Neuronal apoptosis in CA1 region was detected by Hoechst 33342 staining. White arrowheads showed Hoechst 33342 positive apoptotic cells. Scale bar: 30 μm. (b) Quantitative analysis of Hoechst 33342 positive cells in CA1 region under 200x fields. (c) Expression of Bcl-2 and Bax in hippocampus tissues examined by Western blot. ((d), (e)) Quantitative analysis of the expression of Bcl-2 and Bax normalized with GAPDH using data obtained from the different blots. versus control, and versus model, and versus sham-EA.

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