Autophagic Cell Death by Poncirus trifoliata Rafin., a Traditional Oriental Medicine, in Human Oral Cancer HSC-4 Cells

Figure 3

Effects of MEPT on the induction of apoptosis. HSC-4 cells were treated with 0, 25, 50, 100, or 150 μg/mL of MEPT for 24 h or 30 nM paclitaxel for 24 h. Cells were stained with ANNEXIN V and 7-AAD and analyzed by flow cytometry. Early apoptotic cells were stained by ANNEXIN V, but not by 7-AAD (lower right quadrant, Q4), whereas late apoptotic cells were stained by both of ANNEXIN V and 7-AAD (upper right quadrant, Q2).