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Evidence-Based Complementary and Alternative Medicine
Volume 2015, Article ID 397181, 11 pages
http://dx.doi.org/10.1155/2015/397181
Research Article

Potent Antiproliferative Effect on Liver Cancer of Medicinal Plants Selected from the Thai/Lanna Medicinal Plant Recipe Database “MANOSROI III”

1Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
2Manose Health and Beauty Research Center, Chiang Mai 50200, Thailand
3Department of Biotechnology and Material Chemistry, Nihon University Junior College, Chiba 274-8501, Japan
4College of Science and Technology, Nihon University, Tokyo 101-8308, Japan
5Akihisa Medical Clinic, 1086-3 Kamo, Sanda-shi, Hyogo 669-1331, Japan
6Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

Received 31 March 2015; Accepted 25 May 2015

Academic Editor: Shuang-En Chuang

Copyright © 2015 Aranya Manosroi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Thai/Lanna medicinal plant recipes have been used for the treatment of several diseases including liver cancer. In this study, methanolic extracts (MEs) of 23 plants were tested for antiproliferative activity on human hepatoma cell line (Hep G2) by the sulforhodamine B (SRB) assay. Nine MEs with potent antiproliferative activity (IC50 < 100 µg/mL) were obtained and further semipurified by liquid/liquid partition extraction. The semipurified fractions were tested for the antiproliferative and antioxidative activities. ME of Stemona collinsae and the semipurified extract and methanol-water fraction (MF) of Gloriosa superba gave the highest antiproliferative activity on HepG2 which were 4.79- and 50.07-fold cisplatin, respectively. The semipurified fractions showed an increased antiproliferative activity. MF of Caesalpinia sappan and HF of Senna alata showed the highest free radical scavenging and metal chelating activities, respectively. The compound in -hexane fraction (HF) of Ventilago denticulata which showed an increase in antiproliferative activity comparing to its ME was isolated and identified as emodin. This study has demonstrated the potential of the ME from S. collinsae, MF from G. superba, and emodin isolated from V. denticulata, for further development as an antiliver cancer agent.