Auraptene, a Major Compound of Supercritical Fluid Extract of Phalsak (Citrus Hassaku Hort ex Tanaka), Induces Apoptosis through the Suppression of mTOR Pathways in Human Gastric Cancer SNU-1 Cells

Figure 3

Nuclear Hoechst 33342 staining, cell cycle analysis, and determination of mitochondrial membrane potential. (a) Cells were treated with the indicated concentration of auraptene (25–100 μM) for 24 h and then stained with Hoechst 33342. Stained cells were visualized under a fluorescence microscope. (b) Mitochondrial membrane potential was determined by FACS caliber after being treated with given doses of auraptene. (Data represent the means SD of at least four independent experiments. indicate statistically significant differences versus control group.)

(a)

(b)